October 24, 2025 (v1) Dataset Open Enterococcus faecalis genome sequences_illumina sequencing

Genome sequences of Enterococcus faecalis strains V583 and B1 used in Kapitan et al. doi: 10.1101/2024.09.11.612452, generated by nanopore sequencing Bacterial DNA Isolation and Whole Genome Sequencing:Genomic DNA was isolated using the Qiagen Genomic-tip 100/G or DNeasy Blood & Tissue Kit according to the manual for gram positive bacteria. DNA was quantified, fragmented, and used to prepare sequencing librariers using the TruSeq Nano kit (Illumina. Sequencing libraries were sequenced on Illumina Nextseq creating 300bp paired-end reads. Sequencing coverage was 100-fold. Please read the file "Sample ID and strain name_for publication" for information on sample IDs and repsective strain designations.本数据集包含Kapitan等人（doi: 10.1101/2024.09.11.612452）研究中使用的粪肠球菌（Enterococcus faecalis）菌株V583与B1的基因组序列，该序列通过纳米孔测序（nanopore sequencing）获得。 细菌DNA提取与全基因组测序： 依照革兰氏阳性菌操作手册，采用Qiagen Genomic-tip 100/G试剂盒或DNeasy血液与组织试剂盒提取基因组DNA。对DNA进行定量、片段化处理后，使用TruSeq Nano试剂盒（Illumina）构建测序文库。测序文库在Illumina Nextseq平台上进行测序，生成300bp双端reads，测序覆盖度为100倍。 如需获取样本ID及其对应菌株命名的相关信息，请查阅文件「样本ID与菌株名称_待发表」（Sample ID and strain name_for publication）。