Fluorescence Microscopy Images from CBMI

Intracellular organelle networks such as the endoplasmic reticulum (ER) network and the mitochondrial network serve crucial physiological functions. Morphology of these networks plays critical roles in mediating their functions.Accurate image segmentation is required for analyzing morphology of these networks for applications such as disease diagnosis and drug discovery. Deep learning models have shown remarkable advantages in accurate and robust segmentation of these complex network structures. To support the training and testing of deep learning segmentation models, we construct two fluorescence image datasets, ER and MITO, for the ER network and the mitochondrial network, respectively. We provide manual segmentation for these organelle networks in binary masks. The datasets have been used to evaluate and compare performance of the methods proposed in our article “Heuristic Optimization of Deep Learning Models for Segmentation of Intracellular Organelle Networks”.