Impact of Pleistocene climate on diversification and distribution of congeneric freshwater crustacean species

AimThe climatic oscillations of the glacial-interglacial cycles significantly influenced species diversification, playing an essential role in shaping the current patterns of biodiversity. However, the effects of the Pleistocene climate on species diversification and their current distribution are poorly understood for the Southern Hemisphere and some taxonomic groups, such as invertebrates.  Here, we explore the role of Pleistocene climate changes in the diversification and distribution of four species of South American freshwater crustaceans.
LocationSouthern South America. 
TaxonGenus Aegla (Aeglidae).
MethodsWe performed phylogenetic analyses, estimated genetic diversity, and neutrality indices for Aegla alacalufi, Aegla neuquensis, Aegla platensis, and Aegla uruguayana using nuclear and mitochondrial sequences. We used the mitochondrial genes to estimate divergence times, calculate historical demography, and reconstruct the ancestral area range. We also estimated current ..., Genetic and species occurrence data were obtained from the published literature, GenBank (http://www.ncbi.nlm.nih.gov/genbank) and Dryad (https://datadryad.org/stash), and encompass most of the distribution areas of each species.
We performed phylogenetic analyses, estimated genetic diversity, and neutrality indices for Aegla alacalufi, Aegla neuquensis, Aegla platensis, and Aegla uruguayana using nuclear and mitochondrial sequences. We used the mitochondrial genes to estimate divergence times, calculate historical demography, and reconstruct the ancestral area range. We also estimated current and past potential distributions using ecological niche modeling., , # Dataset Impact of Pleistocene Climate on Diversification and Distribution of Congeneric Freshwater Crustacean Species

[https://doi.org/10.5061/dryad.76hdr7t5n](https://doi.org/10.5061/dryad.76hdr7t5n)

## Description of the data and file structure

We performed phylogenetic analyses, estimated genetic diversity, and neutrality indices for *Aegla alacalufi*, *Aegla neuquensis*, *Aegla platensis*, and *Aegla uruguayana* using nuclear and mitochondrial sequences. We used the mitochondrial genes to estimate divergence times, calculate historical demography, and reconstruct the ancestral area range. We also estimated current and past potential distributions using ecological niche modeling. Genetic and species occurrence data were obtained from the published literature, GenBank ([http://www.ncbi.nlm.nih.gov/genbank](http://www.ncbi.nlm.nih.gov/genbank)) and Dryad ([https://datadryad.org](https://datadryad.org)), and are cited in the manuscript.

### Files and variables

#### File: Data\_se...,

研究目的：冰期-间冰期旋回的气候振荡显著驱动物种分化，在塑造当代全球生物多样性格局中发挥核心作用。然而，针对南半球及无脊椎动物等部分类群，更新世（Pleistocene）气候对物种分化及其当前分布的影响仍未得到充分解析。本研究旨在探讨更新世气候变化对4种南美淡水甲壳类物种分化与分布的调控作用。
研究区域：南美南部。
研究类群：阿格螯虾属（Aegla），螯虾科（Aeglidae）。
方法：本研究针对阿拉卡卢夫阿格螯虾（*Aegla alacalufi*）、内乌肯阿格螯虾（*Aegla neuquensis*）、普拉滕斯阿格螯虾（*Aegla platensis*）及乌拉圭阿格螯虾（*Aegla uruguayana*），利用核基因与线粒体序列开展系统发育分析，估算遗传多样性与中性检验统计量；通过线粒体基因估算物种分化时间、解析历史种群动态，并重建祖先分布区范围；此外还采用生态位模型估算当前与冰期时期的潜在分布范围。本研究的遗传数据与物种分布点数据获取自已发表文献、GenBank（http://www.ncbi.nlm.nih.gov/genbank）及Dryad数据仓库（https://datadryad.org/stash），覆盖各物种的绝大多数分布区域。
我们针对上述四种阿格螯虾再次开展了基于核基因与线粒体序列的系统发育分析，估算遗传多样性与中性检验统计量；利用线粒体基因估算分化时间、解析历史种群动态并重建祖先分布区范围；同时通过生态位模型估算当前及过去的潜在分布范围。
# 数据集：更新世气候对同属淡水甲壳类物种分化与分布的影响
[https://doi.org/10.5061/dryad.76hdr7t5n](https://doi.org/10.5061/dryad.76hdr7t5n)

## 数据与文件结构说明
我们针对*Aegla alacalufi*、*Aegla neuquensis*、*Aegla platensis*及*Aegla uruguayana*，利用核基因与线粒体序列开展系统发育分析，估算遗传多样性与中性检验统计量；通过线粒体基因估算物种分化时间、解析历史种群动态，并重建祖先分布区范围；此外还采用生态位模型估算当前与冰期时期的潜在分布范围。本研究的遗传数据与物种分布点数据获取自已发表文献、GenBank（http://www.ncbi.nlm.nih.gov/genbank）及Dryad数据仓库（https://datadryad.org），相关数据来源已在论文中注明。

### 文件与变量
#### 文件：Data_se...,