Identification of endothelial derived factors that promote metastasis I

To identify endothelial-derived factors that may instructively drive cancer metastasis, we generated a new mouse model that allows endothelial-specific ribosomal tagging. We crossed the RiboTag (Rpl22fl/flHA) mice with the endothelial inducible Cre line Cdh5(PAC)-CreERT2 mice. Endothelial derived ribosome-associated mRNA-sequencing was performed from highly metastatic B16F10 and poorly metastatic B16F0 tumours. RNASeq and differential gene expression analysis of the tumor endothelium in highly versus poorly metastatic tumors by endothelial cell specific ribosomal tagging in Rpl22fl/flHA;Cdh5(PAC)-CreERT2 mice