Runx3 restoration regresses K-Ras–activated mouse lung cancers and inhibits recurrence

Comparision of the gene expression profiles of the RUNX3 Knock out and RUNX3 restored cells. Overall design: we obtained immortalized cell lines from KPRL/F-TAM(-) lung cancers (KPR-). Treatment of KPR- cell lines with 4-hydroxytamoxifen (4-OHT) restored Runx3, generating KPRrestored cell lines. We confirmed that Runx3 was restored at 8 h after 4-OHT treatment. To identify genes regulated by Runx3 in the K-Ras–activated lung cancer cells, we performed mRNA sequencing (RNA-seq) in KPR- cells and KPRrestored cells.