Investigation of Bach2 function by regulating inflammatory response and heme-iron homeostasis of bone marrow macrophage.. Mus musculus

Macrophages are central in regulating iron homeostasis. Transcription repressor Bach2 regulates by heme. Here we investigated the relationship between heme-regulated Bach2 and macrophage in bone marrow. We identified RFP-positive and negative macrophage were in bone marrow. We found that RFP-positive macrophage related with iron-heme homeostasis maintenance and RPF-negative population related with immune response. In RFP positive macrophage, we also found the lysosomal heme transporter hrg-1 was Bach2 direct target gene. Our results suggest that the function of the bone marrow macrophage alters according to expression of Bach2. Overall design: Gene expression in bone marrow macrophages cells of Bach2-td RFP reporter mice was measured. 3 independent mice ware used for each genotype.