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CRISPR-Cas9 Engineering in Primary Cell Protocol

NIAID Data Ecosystem2026-05-10 收录

CRISPRi基因编辑

冠状病毒宿主因子

数据链接：

https://immport.org/shared/study/SDY1390 数据链接链接失效反馈

官方服务：

资源简介：

Ex vivo genome editing of primary human T cells can be used to identify viral host factors

应用场景：

创建时间：

2025-10-30

CRISPRi基因编辑

遗传开关设计

We present a novel genetic switch we term SWITCHER based on a floxed wild-type Cre-construct representing a CRISPR-inducible and self-limiting kill switch. By leveraging CRISPR/Cas12a-mediated crRNA-a

NIAID Data Ecosystem40

CRISPR RNA-seq from HepG2 (ENCSR194SPW)

CRISPRi基因编辑

肝癌基因表达

Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV07-B) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov

NIAID Data Ecosystem30

Frequency of INDEL formation in mice using gRNA synthesized from pU6T7 expression vectors.

CRISPRi基因编辑

INDEL突变频率

Frequency of INDEL formation in mice using gRNA synthesized from pU6T7 expression vectors.

NIAID Data Ecosystem20

CRISPR RNA-seq from K562 (ENCSR234FWR)

CRISPRi基因编辑

转录组分析

RNA-seq on K562 cells treated with a CRISPR gRNA against RPL28. (RPL28-BGKcLV15) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Re

NIAID Data Ecosystem40

File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

CRISPR-Cas9 Engineering in Primary Cell Protocol

资源简介：

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