Genome assembly and annotation of <i>Lobelia seguinii</i>

This dataset corresponds to the data in the manuscript: A chromosome-level genome of<i> Lobelia seguinii</i> provides insights into the evolution of Campanulaceae and the lobeline biosynthesis, which was submitted to Genomics.This dataset includes the chromosome-level assembly (fasta) and annotations (cds, pep, gff3). Genome sequencing was carried out for <i>Lobelia seguinii</i> using HiFi, Illumina, and Hi-C sequencing on fresh leaves that were frozen with liquid nitrogen. After DNA extraction, HiFi sequencing was performed using the PacBio Sequel II platform, while Illumina and Hi-C sequencing were conducted on the NovaSeq 6000 platform. The HiFi sequencing reads were assembled using Hifiasm (v0.16.1-r375), and the Hi-C sequencing reads were mapped to the assembly using Juicer (v1.6). After obtaining the genome assembly file (<b><i>Lobelia_seguinii_genome.fa</i></b>), ab initio, homology-based, and transcriptome-based approaches were used for the annotation of the <i>L. seguinii</i> genome (<b><i>Lobelia_seguinii_gene.cds</i></b>, <b><i>Lobelia_seguinii_gene.pep</i></b>, <b><i>Lobelia_seguinii_genome.gff3</i></b>).