Characterization of the crosstalk between Notch signaling and hypoxia. Characterization of the crosstalk between Notch signaling and hypoxia

The Notch signaling pathway regulates several differentiation and developmental processes. Reduction of oxygen availability, a condition referred to as hypoxia, impacts on the Notch response. Here we made use of RNA-Seq and ChIP-Seq analysis to investigate the Notch/hypoxia crosstalk at genomic level. Overall design: Mouse pre-T cells (Beko) were treated with the DMOG or kept under low oxygen concentration to induce hypoxia. Effects on gene expression were investigated by RNA-Seq and RBPJ occupancy was analyzed by ChIP-Seq.