Single-cell RNA sequencing data of human craniopharyngioma tumor tissue

The craniopharyngioma tumor specimen was digested immediately after surgical resection, and a single-cell library was prepared using 10x Genomics. The obtained sequencing data were processed and analyzed using Cell Ranger. The cell suspension obtained from the resected tumor specimens was processed according to 10×Genomics specifications, using the 10×Chromium Next GEM single-cell 3ʹ reagent kit and library construction kit. The cell suspension was processed in the Chromium Controller to create gel beads in emulsion (GEMs), incubated at a specific temperature to generate cDNA containing barcodes and Unique Molecular Identifiers (UMIs). Subsequently, the cDNA was washed, amplified, and its quality assessed, then enzymatically fragmented and size-selected, followed by adapter ligation and indexed PCR amplification. Finally, after quantification and quality assessment of the library, sequencing was performed on the NovaSeq 6000.