Kkv and Chs2 chimeras

(A) Schematics of Kkv and Chs2 showing the different domains and the position of the catalytic domain (QRRRW) and the WGTRE domain. Two different chimeric proteins between Kkv and Chs2 were generated as indicated. (B-J) Embryos and proventriculus of the indicated genotypes are shown, stained as indicated. (B-D) The chimeras cannot rescue the absence of chitin in a kkv mutant background. (E-F) The chimeras cannot rescue the absence of chitin in the PM in a Chs2 mutant background (yellow arrows point to the absence of PM in the proventricular lumen). (G,H) The chimeras localise intracellularly in embryonic tissues, largely colocalising with the ER marker KDEL.(I,J) The chimeras localise diffusely in PR cells. Scale bars B-D,G,H 10μm; E,F,I,J 20 μm.