Fungal Leaf Phyllosphere Microbiome Treated with Copper- and Sulphur Based Fungicides. (ITS Sequences)

Here we investigate the impacts of two common fungicide types: copper- and sulphur-based, on the phyllosphere microbiome of grapevines. Potted Vitis vinifera (Shiraz grapevines kept in a semi-controlled, semi-natural environment were treated with high and low doses of the two fungicides and were compared to a water spray control. PMA-Seq targeting the 16S rRNA gene (V4) for bacteria, and the ITS gene (ITS1) for fungi, allowed us to observe the changes in diversity and richness of the phyllosphere microbiome over three weeks post-treatment.