Kras mutation-induced gene expression in mouse MEF. Kras mutation-induced gene expression in mouse MEF

The oncogenic activations of KRAS, or its surrogates, occur frequently in human cancers. The alterations in the transcriptional profiles of genes in response to KRAS activation were examined using mouse embryonic fibroblasts. Overall design: Mouse embryonic fibroblasts (MEFs) with or without the Loxp-Stop-Loxp-KrasG12D/+ allele were immortalized with the SV40 large T antigen and subsequently modified by Cre-recombinase-induced activation of oncogenic KrasG12D. The gene expression profiling in the tumorigenic KrasG12D MEFs and non-tumorigenic Kraswt MEFs were analyzed by RNA-seq.