The inhibitory rates on the SGC-7901 cells treated with TSN or 5-FU for different time

Supplementary data 1. The SGC-7901 cells were seeded on plates filled with RPMI1640 medium at a density of 1×104 cells/well in 96-well plates, and cultivated for 24 h to make them adhere on plates, and then treated with TSN and 5-fluorouracil (5-FU) respectively to reach a final concentration of 0, 20, 40, 60, 80, 100 nmol/L for 24, 48 and 72 h. Subsequently，the cells were incubated with 0.5μg/μL 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl- tetrazolium bromide (MTT) at 37℃ for 4h. The absorbance was measured using a Multi-well Plate Reader (Bio-Rad, Hercules, California, USA) at the wavelength of 570 nm. Cell inhibitory rate was calculated according to the formula: (1-absorbance of the cells treated with drug/absorbance of untreated control cells) ×100%. Supplementary data 2. The SGC-7901 were grown on Histogrip coated glass coverslips (Invitrogen, Carlsbad, CA, USA) in plates at a density of 1×104 cells/60-mm dish, and separately treated with TSN (0, 50, 70, 90 nmol/L) and 5-FU (70 nmol/L) for 48 h. Afterwards, the medium was discarded, 1 mL phosphate buffer solution (PBS) and 200 µL acridine orange solution (0.5 μg/μL) were added to the wells of the plates, respectively, then the plates were kept at room temperature for 3~5 min. The cellular morphologies were observed, and photographed using a laser confocal microscopy (Leica TCS SP8, Germany).

补充数据1。将SGC-7901细胞以1×10^4个/孔的密度接种于含RPMI1640培养基的96孔板中，培养24小时待细胞贴壁，随后分别用TSN与5-氟尿嘧啶（5-FU）处理，使终浓度分别为0、20、40、60、80、100 nmol/L，处理时间为24、48、72小时。随后，将细胞与0.5μg/μL的3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）于37℃下孵育4小时。采用多孔板读数仪（Bio-Rad，美国加利福尼亚州赫拉克勒斯）在570nm波长下检测吸光度。细胞抑制率按以下公式计算：(1-药物处理组细胞吸光度/未处理对照组细胞吸光度)×100%。
补充数据2。将SGC-7901细胞以1×10^4个/60mm培养皿的密度接种于包被有Histogrip的盖玻片（Invitrogen，美国加利福尼亚州卡尔斯巴德）中进行培养，分别用浓度为0、50、70、90 nmol/L的TSN以及70 nmol/L的5-FU处理48小时。随后弃去培养基，向各孔中分别加入1mL磷酸盐缓冲液（PBS）与200μL吖啶橙染液（0.5μg/μL），室温孵育3~5分钟。采用激光共聚焦显微镜（Leica TCS SP8，德国）观察细胞形态并拍照记录。