C2C12 expressing desmin: WT vs Mutations R406W and I451M. C2C12 expressing desmin: WT vs Mutations R406W and I451M

C2C12 cell lines were genetically engineered to stably express WT human Desmin or the Desmin mutant R406W or I451M. Non-modified C2C12 cells were used as control. The goal was to determine the effect of different desmin mutation on the gene expression in a myoblast cell line. Overall design: Three experimental conditions: WT vs control (1 biological sample), R406W vs Control (1 biological sample), I451M vs control (2 biological samples)