Ischemia promotes hypertrophic nerve trunk formation and enteric neuron cell death in Hirschsprung's disease: Source data

Items Annotation ST-cluster_distrition.csv A table containing the number of ST-clusters across samples DEGs_C10_v_restCMusc.csv A table containing the result of DEG analysis for the comparison between ST cluster C10 and other muscular ST clusters (C8 and C9) DEGs_C13_v_C12.csv A table containing the result of DEG analysis for the comparison between ST cluster C13 (HNTs) and C12 (ganglia). DEGs_ST-cluster_total_marker.csv A table containing the markers for each ST cluster. DEGs_iMusc_ganglia_v_nMusc_ganglia.csv A table containing the result of DEG analysis for the comparison between ST iMusc_ganglia and nMusc_ganglia. DEGs_sc_all_markers.csv A table containing the markers of single-cell populations. DEGs_pseudotime_CLDN1.csv A table containing the results of gene differentially expressed during the differentiation from GREM1+ FLCs to CLDN1+ FLCs. Gene_set_score_for_C12_sub.csv A table containing the results of gene set score in the sub-population of ganglia (C12). scRNA-seq_reference_raw.RDS A Seurat object containing the raw count and meta matrix of the single-cell reference ST-seq_raw_count.txt.gz A raw count matrix of ST-seq data ST-spots_meta_info.txt A table containing the metainformation of ST-seq data Object_of_bulk_RNA-seq.RDS A Seurat object used to reproduce the results of gene set scores in bulk RNA-seq. ST-spots_cell_abundance.txt A table containing the abundance of single-cell populations in each ST-spot. Cell_trajectory_of_FLCs.RDS A Seurat object used to reproduce the results of the cell trajectory of fibroblast-like cells. p_value_scRNA-seq_abundance.xlsx A table containing the p-value of cell abundance across groups for single-cell populations CIBERSORT_CPM_count_matrix.txt A table containing the CMP matrix of bulk RNA-seq data for CIBERSORT CIBERSORT_ST_raw_matrix.txt A table containing the raw count matrix of ST-seq data for CIBERSORT scRNA-seq_distribution.csv A table containing the distribution of single-cell populations across the sample DEGs_FLC_APOD_across_group.csv A table containing the DEGs in the comparison among APOD+ FLCs in different groups Gene_set_score_for_sc_Pericyte.csv A table containing the results of gene set score in the single-cell population of pericytes Gene_set_score_for_sc_Endo_score.csv A table containing the results of gene set score in the single-cell population of endothelial cells ENC_FLC_vessel_interaction.RDS A Cellchat object used to reproduce the results of cell-cell interaction among ENCs, glial cells, APOD+ FLCs, CLDN1+ FLCs, perictyes, and vascular endothelial cells. Metainfor_FASTQ1.xlsx Metainformation of FASTQ files Metainfor_FASTQ2.xlsx Metainformation of FASTQ files

注释条目 ST-cluster_distrition.csv：记录各样本中ST簇数量的表格
DEGs_C10_v_restCMusc.csv：包含ST簇C10与其余肌肉型ST簇（C8、C9）组间差异表达基因（Differentially Expressed Gene, DEG）分析结果的表格
DEGs_C13_v_C12.csv：包含ST簇C13（HNTs）与C12（神经节）组间差异表达基因分析结果的表格
DEGs_ST-cluster_total_marker.csv：包含各ST簇特异性标记基因的表格
DEGs_iMusc_ganglia_v_nMusc_ganglia.csv：包含ST型iMusc_ganglia与nMusc_ganglia组间差异表达基因分析结果的表格
DEGs_sc_all_markers.csv：包含各单细胞群体特异性标记基因的表格
DEGs_pseudotime_CLDN1.csv：包含从GREM1+成纤维细胞样细胞（Fibroblast-like Cells, FLCs）向CLDN1+ FLCs分化过程中差异表达基因的分析结果表格
Gene_set_score_for_C12_sub.csv：包含神经节亚群（C12）基因集得分结果的表格
scRNA-seq_reference_raw.RDS：一个Seurat对象，包含单细胞参考数据集的原始计数矩阵与元数据矩阵
ST-seq_raw_count.txt.gz：空间转录组测序（ST-seq）数据的原始计数矩阵
ST-spots_meta_info.txt：包含ST-seq数据位点元信息的表格
Object_of_bulk_RNA-seq.RDS：一个Seurat对象，用于复现批量RNA-seq分析中的基因集得分结果
ST-spots_cell_abundance.txt：包含每个ST位点中各单细胞群体丰度的表格
Cell_trajectory_of_FLCs.RDS：一个Seurat对象，用于复现成纤维细胞样细胞（FLCs）的细胞轨迹分析结果
p_value_scRNA-seq_abundance.xlsx：包含各单细胞群体在各组间细胞丰度P值的表格
CIBERSORT_CPM_count_matrix.txt：包含用于CIBERSORT分析的批量RNA-seq数据每百万reads计数（Counts Per Million, CPM）矩阵的表格
CIBERSORT_ST_raw_matrix.txt：包含用于CIBERSORT分析的ST-seq数据原始计数矩阵的表格
scRNA-seq_distribution.csv：包含各单细胞群体在各样本中分布情况的表格
DEGs_FLC_APOD_across_group.csv：包含不同组别中APOD+ FLCs组间差异表达基因的表格
Gene_set_score_for_sc_Pericyte.csv：包含周细胞（Pericyte）单细胞群体基因集得分结果的表格
Gene_set_score_for_sc_Endo_score.csv：包含内皮细胞（Endothelial Cell）单细胞群体基因集得分结果的表格
ENC_FLC_vessel_interaction.RDS：一个Cellchat对象，用于复现神经元外被细胞（ENC）、胶质细胞、APOD+ FLCs、CLDN1+ FLCs、周细胞与血管内皮细胞间的细胞互作分析结果
Metainfor_FASTQ1.xlsx：FASTQ文件的元数据信息
Metainfor_FASTQ2.xlsx：FASTQ文件的元数据信息