Mitochondrial DNA mutations induce supression of nuclear encoded mitochondrial Genes in skeletal muscle

To identify and analyze mtDNA mutation-responsive genes, a comparison of gastrocnemius muscle tissues from WT (control) and D257A mice was conducted. We examined changes in gene expression in the muscle associated with mtDNA mutations. 1) Types of experiments: a) Effect of mitochondrial DNA (mtDNA) mutations b) Effect of aging c) WT (13 month-old Polg+/+ mice) vs. D257A (13 month-old PolgD257A/D257A) mice 2) Experimental factors: a) Type of gene: PolgD257A/D257A b) Time (age) 3) Number of hybridizations in the study: ~10 4) A common reference RNA was not used. 5) Quality control measures were not used. No replicates were done. Dye swap was not used.