Catalysis of Ac-DEVD-AMC by caspase-3 (dataset formatted for analysis by interferENZY)

<strong>Main description</strong> This dataset depicts the catalysis of the fluorogenic substrate Ac-DEVD-AMC to the fluorescent substrate AMC by recombinant purified caspase-3, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in <em>Biophysical Chemistry 252 (2019) 106193</em> (https://doi.org/10.1016/j.bpc.2019.106193), and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis. <strong>Dataset organization</strong> Line 1: Tab-separated initial concentrations of substrate Ac-DEVD-AMC in micromolar (µM) concentration Line 2: Concentration of enzyme (1 U) Line 3: Units of time Line 4: Units of concentration for substrate values and measurements Line 5: Dataset name Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1 <strong>Contact information:</strong> Maria Filipa Pinto ([e-mail])<br> Pedro M. Martins ([e-mail]) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]【数据集主描述】本数据集记录了重组纯化的半胱天冬氨酸蛋白酶-3（caspase-3）催化荧光底物Ac-DEVD-AMC转化为荧光产物AMC的反应过程，其中酶浓度固定，初始底物浓度呈梯度变化。该数据集最初发表于《Biophysical Chemistry》2019年第252卷，文章编号106193（https://doi.org/10.1016/j.bpc.2019.106193），后被用作在线工具interferENZY（一款用于酶活实验验证与标准化动力学分析的网页端工具，详细信息请访问https://interferenzy.i3s.up.pt）的研究案例。为适配该工具的分析需求，本数据集已转换为当前格式：即interferENZY分析所需的制表符分隔*.txt输入文件。 【数据集组织格式】 第1行：制表符分隔的初始底物Ac-DEVD-AMC浓度，单位为微摩尔（µM） 第2行：酶浓度（1 U） 第3行：时间单位 第4行：底物浓度与检测值的浓度单位 第5行：数据集名称 第6行及后续行：制表符分隔的反应进程曲线列对（时间，产物浓度），对应第1行中所列的各初始底物浓度。 【联系方式】 玛丽亚·菲利帕·平托（[e-mail]） 佩德罗·M·马丁斯（[e-mail]） 葡萄牙波尔图大学健康研究与创新研究所（i3S），地址：Rua Alfredo Allen, 208, 4200-135 波尔图，葡萄牙。 联系电话：[phone]