Mendelian ratio of offspring from GD11.5 through PND3, resulting from the Slc39a8(+/neo)×Slc39a8(+/neo) cross.

aNS, Not significant (P>0.05). Chi-square analysis with two degrees of freedom was used to calculate the P-values, assuming the genotype distribution follows the expected Mendelian ratio.bOut of a total of 94 Slc39a8(neo/neo) homozygotes genotyped, one pup survived beyond 48 h; this male was runted and actually survived for more than 4 months but failed to breed; no offspring could be obtained from this single Slc39a8(neo/neo) survivor.