Amur grayling genome files

Graylings (<i>Thymallus</i>) represent one of the earliest diverged salmonid subfamilies and provide crucial insights into the evolutionary consequences of the salmonid-specific whole genome duplication event (Ss4R). Here, we present the first chromosome-level genome assembly of the Amur grayling (<i>Thymallus grubii</i>), an economically and ecologically important species endemic to East Asian freshwater systems. The final assembly spans 1,754.51 Mb with 46 pseudo-chromosomes, achieving a contig N50 of 3.03 Mb and anchoring 99.97% of sequences to chromosomes. The genome contains 31,867 protein-coding genes with 97.58% functionally annotated. Repetitive sequences comprise 52.64% of the genome, dominated by DNA transposons (22.06%). BUSCO analysis indicates 97.9% completeness for conserved Actinopterygii genes. This high-quality genome assembly provides an essential resource for salmonid comparative genomics, aquaculture breeding programs, and conservation genetics of this economically important species.