Microarray gene expression in RKO cell line under low replication stress

RKO cells were treated with low doses of aphidicolin (0,2µM) that inhibit replicative DNA polymerases and induce a mild replication stress. Expression data were analysed on control cells (DMSO), 16h of treatment (t0) and after release in complete new culture medium of 13h (N+1) using microarray (affymetrix Clarion S) We used microarray to analyse the impact of low replication stress on gene expression comparing DMSO or aphidicolin-treated cells at the end of the treatment (t0) and in daughter cells released from the replication stress (N+1). Triplicates of total RNA was extracted from the same amount of exponentially growing RKO cells in different conditions (DMSO or aphidicolin, t0 or N+1) and hybridized on Affymetrix Microarrays.