Normalized Correction Efficiencies.
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Unsynchronized HCT116-19 cells were harvested and electroporated at a concentration of 5x105cells/100ul with 2ug of the indicated CRISPR/Cas9 (2C, 3C, 5C and empty pX458 vector) plus 1.35ug of either 72NT or 72 PM. Following electroporation, transfection efficiency was determined after 24 hours of incubation by the percentage of total viable eGFP+ cells in the population. The normalized correction efficiency was determined after 48 hours of incubation as the percentage of total viable eGFP+ cells in the population divided by the transfection efficiency. Standard Error was calculated from two sets of data points generated over two separate experiments.Normalized Correction Efficiencies.
创建时间：
2015-12-03
