Supplementary data of Persimmon Vinegar Ameliorates Cisplatin-Induced Dysbiosis Through Gut Microbiota Reprogramming and Suppressing Intestinal Inflammation

Cisplatin is an effective chemotherapy drug, but its use is limited by intestinal mucositis, dysbiosis, and liver toxicity linked to oxidative stress. This study investigated the protective effects of persimmon vinegar (Diospyros kaki L.) in BALB/c mice exposed to cisplatin.
Twenty-eight male mice were divided into seven groups, including controls and persimmon vinegar treatments (26, 52, and 104 mg/kg BW). Treatments were given orally before and after cisplatin induction. Evaluations included stool parameters, histopathology, and oxidative stress markers (SOD and MDA).
Cisplatin caused severe diarrhea, oxidative imbalance (increased MDA, decreased SOD), and tissue damage in the colon and liver. Persimmon vinegar significantly improved these conditions, with the 52 mg/kg BW dose showing the strongest protective effect, comparable to standard treatments.
These effects are likely due to antioxidant and anti-inflammatory properties of phenolic compounds and acetic acid, as well as modulation of the gut–liver axis. Overall, persimmon vinegar shows potential as a supportive therapy to reduce cisplatin-induced toxicity.