HeLa_H2BmCherry_GFPtubulin_Mitotracker

HeLa cell expressing H2B-mCherry, GFP-tubulin, stained with MitoTracker-DeepRed, acquired on Spinning Disk CSU-W1 (https://www.epfl.ch/research/facilities/ptbiop/equipment/visitron-csu-w1/) with an objective 60X N.A 1.42, Orca Flash 4.0 sCMOS camera. The time-lapse serie was cropped, time-lapse step reduced from 1 min to 3 min and rescaled to 8-bit (to reduce dataset size)本数据集的成像样本为表达H2B-mCherry、GFP-微管蛋白（GFP-tubulin）的海拉（HeLa）细胞，经MitoTracker-DeepRed染色；图像采集采用Spinning Disk CSU-W1型转盘式共聚焦显微镜（配套链接：https://www.epfl.ch/research/facilities/ptbiop/equipment/visitron-csu-w1/），搭配60倍、数值孔径（N.A）1.42的物镜及Orca Flash 4.0 sCMOS相机。该延时成像序列经裁剪处理，将延时步长从1分钟缩减至3分钟，并缩放至8位图像格式以减小数据集体积。