Preliminary Venomic Characterization of the Venom of Phalotris lemniscatus (Serpentes, Colubridae)

This dataset correspond to the proteomic analysis of the article entitle “Preliminary Biochemical and Venomic Characterization of the Venom of Phalotris lemniscatus (Serpentes, Colubridae)” sending to Current topics in medicinal chemistry. The data set include the raw files of each band of mass spectroscopy and the database used to identify the proteins. To observe the SDS page, please refer to the article. “Protein bands were excised and sent to the Spectroscopy and Biophysics Core, University of Nebraska, Lincoln (via Science Exchange) to in-gel trypsin digestion and peptide fragmentation by LC-MS/MS. The instrument was an LTQ Velos Pro (Thermo Scientific) equipped with dual pressure ion-trap. Raw data obtained by proteomic analysis was analyzed using MaxQuant software [30–32] with the default parameters (false discovery rate 0,01). As a sequence database to match for protein identification, a fasta file download from Uniprot was used. Database was constituted by all the reviewed sequenced proteins of snakes, around 2500 proteins from Swiss-Prot database (taxonomy:"Serpentes (snakes) [8570]" AND reviewed:yes)”.

本数据集对应已投稿至《Current topics in medicinal chemistry》（《药物化学当代议题》）的题为《Phalotris lemniscatus（蛇亚目，游蛇科）毒液的初步生化与蛇毒组学表征》的文章的蛋白质组学分析内容。本数据集包含各质谱条带的原始文件及用于蛋白质鉴定的数据库。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结果请参见该文章。实验流程如下：将蛋白质条带切取后，通过Science Exchange平台送至内布拉斯加大学林肯分校光谱与生物物理核心实验室，进行凝胶内胰蛋白酶消化及液相色谱-串联质谱（LC-MS/MS）肽段碎裂分析；所用仪器为配备双压力离子阱的LTQ Velos Pro（Thermo Scientific）。蛋白质组学分析得到的原始数据采用MaxQuant软件[30–32]进行分析，参数使用默认设置（假发现率为0.01）。用于蛋白质鉴定的序列匹配数据库为从UniProt下载的FASTA文件，该数据库包含Swiss-Prot数据库中所有已审核的蛇类测序蛋白质，共计约2500条，分类学筛选条件为"Serpentes (snakes) [8570]"且已审核：是。