Analysis of two methods of root canal disinfection after preparation for pins: proposal of prosthetic protocol: in vitro study

Abstract Introduction When there is loss of the coronary supports and still further wear due to endodontic treatment, very often, it is necessary to use a intraradical retainer to restore aesthetics and function of lost dental structures. Within this rehabilitation, the aseptic chain which was maintained during endodontics can be broken using some clinical procedures. Aim Test a disinfection protocol by E. faecalis of root canals, in the steps of making an intraradicular retainer, demystifying that the fracture of the aseptic chain and the emergence of root infections are coming from prosthetic rehabilitation. Materials and method 50 single-rooted teeth with complete endodontics were unfilled, they had their conduits prepared for an intraradical retainer and contaminated by Enterococcus. faecalis. The teeth were divided into 3 groups according to the disinfectant solution. G1- saline (n= 10), G2 2.5% sodium hypochlorite (n=20), G3- chlorhexidine 2% (n=20). Then, a disinfection of conduit, drying and analysis of the efficacy of the solution was made. The evaluation of the presence of Enterococcus faecalis was made by cultivating in Brain Heart Infusion broth by the method of turbidity, and subsequent identification by means Agar Bile-Esculin. Statistical analysis were performed using the chi-square method in cross-tabulation, where p <0.0001. Result As a result, it was observed 100% bacteria inhibition in G2 and G3, and bacterial growth of 100% in G1. Conclusion The use of substances assessed in the prosthetic stages of finalizing an intraradical retainer, as filed by this research, is able to maintain the aseptic chain without interfering with success of prosthetic rehabilitation.