BDNF augmentation reverses cranial radiation therapy-induced cognitive decline and neurodegenerative consequences

Spatial transcriptomic profiling was conducted on hippocampal brain sections from mice across four experimental combinations: cranial radiation therapy(RT) with Riluzole (label as Ir-Rz), cranial radiation therapy with vehicle (label as Ir-Veh), control with Riluzole (label as Ctl-Rz), and control with vehicle (label as Ctl-Veh). We used a commercial spatial transcriptomics Multiplexed Error-Robust Fluorescence in situ Hybridization (MERFISH) platform (MERSCOPE, Vizgen, Cambridge, MA), and cell boundary segmentation was completed with custom Cellpose pipeline.