Multicellular modeling of ciliopathy by combining iPS cells and microfluidic airway-on-a-chip technology

Fluid shear stress in the airway chip culture promotes induction of iPS cell-derived multiciliated airway cells (iMCACs) and helps establish the global axis of planar cell polarity. Overall design: Human iPS cell-derived airway epithelial progenitor cells in 3D Matrigel blocks were dissociated on Day 28 and placed on the laminin-coated membrane of the 12-well cell culture inserts or of the upper channel of the devices. In "Healthy donor-iMCACs fluid shear stress(+)", the attached cells 12 hours after seeding were perfused with culture medium using a micro peristaltic pump until Day 42. In "Healthy donor-iMCACs fluid shear stress(-)" and "Healthy donor-iMCACs cell culture insert", the cells were not perfused with culture medium. All the cells in each condition were harvested on Day 42. The transcriptomes of these cells were sequenced with paired-end reads (150-bp) on an Illumina Novaseq 6000 instrument.