Ecology-relevant bacteria drive the evolution of host antimicrobial peptides in Drosophila

Antimicrobial peptides are host-encoded immune effectors that combat pathogens and shape the microbiome in plants and animals. However, little is known about how the host antimicrobial peptide repertoire is adapted to its microbiome. Here we characterize the function and evolution of the Diptericin antimicrobial peptide family of Diptera. Using mutations affecting the two Diptericins (Dpt) of Drosophila melanogaster, we reveal the specific role of DptA for the pathogen Providencia rettgeri and DptB for the gut mutualist Acetobacter. Strikingly, presence of DptA- or DptB-like genes across Diptera correlates with the presence of Providencia and Acetobacter in their environment. Moreover, DptA- and DptB-like sequence predicts host resistance against infection by these bacteria across the genus Drosophila. Our study explains the evolutionary logic behind the bursts of rapid evolution of an antimicrobial peptide family and reveals how the host immune repertoire adapts to changing microbial e..., The dataset was collected through wet lab experimentation and literature review, as described in the manuscript. In brief, bacterial infections were performed with immune mutant Drosophila flies. Immune evolution of fly defence genes was collected from previous studies and newly-published genomic data through sequence-based searches, and the host gene evolution was manually curated with a focus on specific residues of a priori interest., The data are saved in excel file formats, csv file formats, and alignment and phylogeny data are provided in both Geneious proprietary software format, and either .nexus or .genbank as a non-proprietary alternative. Any Geneious files can be viewed with the free version of the software available from the company., # Title of DatasetEcology-relevant bacteria drive the evolution of host antimicrobial peptides in Drosophila

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Fly infection experiment data alongside bioinformatic analyses (alignment, phylogeny, codon-based assessment of evolution) related to the evolution of the Diptera antimicrobial peptide family Diptericin, with a focus on Drosophila fruit flies.

## Description of the data and file structure

The data are provided in folders that relate to the main figures as per the preprint version of the article. In this way, we hope the hierarchical structure of the folders is more intuitive to help readers find the specific data they are interested in related to a specific figure. The figures are titled per the preprint manuscript as this is the published text at time of writing. In the future, the supplementary figure naming format is likely to change from \"Figure XsuppY\", so we encourage users to view the latest version of the open access preprint to sort out any confusion.
see: [https...

抗菌肽（antimicrobial peptides）是宿主编码的免疫效应分子，可对抗病原体并塑造动植物的微生物组。然而，目前学界对宿主抗菌肽组如何适配其微生物组的机制仍知之甚少。本研究对双翅目（Diptera）的双翅肽（Diptericin）抗菌肽家族的功能与进化特征进行了系统解析。我们利用影响黑腹果蝇（Drosophila melanogaster）两种双翅肽（Dpt）的突变体，揭示了DptA特异性对抗病原体雷氏普罗威登斯菌（Providencia rettgeri），而DptB则针对肠道共生菌醋杆菌属（Acetobacter）。值得注意的是，双翅目物种中DptA或DptB同源基因的存在，与其环境中雷氏普罗威登斯菌和醋杆菌属的分布显著相关。此外，在果蝇属中，DptA和DptB的同源序列可预测宿主对这两类细菌感染的抗性。本研究阐明了抗菌肽家族快速爆发式进化背后的进化逻辑，并揭示了宿主免疫组如何适应不断变化的微生物e...

本数据集按照手稿描述的方法，通过湿实验（wet lab experimentation）与文献调研收集得到。简言之，研究采用免疫缺陷突变体果蝇进行细菌感染实验；果蝇防御基因的免疫进化数据来自既往研究与新发表的基因组数据，通过基于序列的检索获取，并针对预先关注的特定氨基酸残基对宿主基因进化进行了人工整理。

数据以Excel格式与CSV格式保存，序列比对与系统发育数据同时提供Geneious专有软件格式，以及非专有的NEXUS格式（.nexus）或GenBank格式（.genbank）作为替代。所有Geneious格式文件均可通过该公司提供的免费版本软件查看。

# 数据集标题：与生态相关的细菌驱动果蝇宿主抗菌肽的进化

本数据集包含与双翅目抗菌肽家族双翅肽进化相关的果蝇感染实验数据与生物信息学分析结果（包括序列比对、系统发育分析、基于密码子的进化评估），研究聚焦于果蝇属昆虫。

## 数据与文件结构说明
本数据集按照文章预印本版本的主图进行文件夹分组，以期通过直观的层级文件夹结构，帮助读者快速定位与特定图表相关的目标数据。本次数据集的图表命名遵循预印本手稿的标注规则（因撰写本说明时预印本为已公开版本）。未来补充图表的命名格式可能会从"Figure XsuppY"变更，因此我们建议用户查阅开放获取预印本的最新版本以消除相关疑惑。详见：[https...