Comparison of MDA methods
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https://edatos.consorciomadrono.es/citation?persistentId=doi:10.21950/HCNDGF
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资源简介：
<p>Comparison of whole metagenome amplification competence by new piPolB-based MDA methods and commercial kits using a mini mock-metagenome containing high-GC sequences. </p>
<p>Mini mock metagenome made up of <i> E. coli, B. subtilis, P. aeruginosa and K. rhizophila genomes.</i> A total of 11 samples were analyzed:</p>
<ul>
<li>unamplified metagenome (NA and NA2)</li>
<li>samples obtained by random-primers MDA (RP-MDA and RP-MDA2)</li>
<li>PrimPol-MDA</li>
<li>new MDA with piPolB as only enzyme</li>
<li>piPolB with a previous DNA alkaline denaturation step (piPolB+D)</li>
<li>new MDA with piPolB and phi29 DNA polymerases (piMDA and piMDA2)</li>
<li>and the latter method with a previous denaturation step (piMDA+D and piMDA+D2)</li></ul>
提供机构：
e-cienciaDatos
创建时间：
2023-03-09
