Comparison of MDA methods

<p>Comparison of whole metagenome amplification competence by new piPolB-based MDA methods and commercial kits using a mini mock-metagenome containing high-GC sequences. </p> <p>Mini mock metagenome made up of <i> E. coli, B. subtilis, P. aeruginosa and K. rhizophila genomes.</i> A total of 11 samples were analyzed:</p> <ul> <li>unamplified metagenome (NA and NA2)</li> <li>samples obtained by random-primers MDA (RP-MDA and RP-MDA2)</li> <li>PrimPol-MDA</li> <li>new MDA with piPolB as only enzyme</li> <li>piPolB with a previous DNA alkaline denaturation step (piPolB+D)</li> <li>new MDA with piPolB and phi29 DNA polymerases (piMDA and piMDA2)</li> <li>and the latter method with a previous denaturation step (piMDA+D and piMDA+D2)</li></ul>