Multiple DC subsets elicit IFN-γ production by CD4+ but not CD8+ TEFF cells.

(A) Sorting strategy used to isolate CD11c+CD11blo and CD11c+CD11bhi DCs, CD11c−Ly6Cint neutrophils (Neut) and CD11c−Ly6Chi monocytes (Mono) from skin 5 days after HSV-1 infection. (B,C) Analysis of IFN-γ+ cells after culture of in vitro activated gDT-II and gBT-II effector cells with 5×104 (B) or increasing numbers (C) of sorted APCs pooled from 10–20 mice. Culture period, 5 h for gBT-I and 18 h for gDT-II cells. Data pooled from 2–3 experiments. (D,E) Analysis of IFN-γ+ TEFF cells cultured with indicated DC subsets pooled from 10–20 mice and sorted from skin (D; 1.5×104) or axillary LNs (E; 5×104) 5 days after infection as in B,C. LC, Langerhans cells. Representative plots from 2–3 independent experiments.