Two RNA-seq studies, one of coding and the other of coding and non-coding RNA for Identification of meningococcal factors required for colonization of epithelial and endothelial cells by high throughput screening

"RNA-Seq analyses were performed in N. meningitidis strain Z5463 by deep sequencing using Ion Torrent technology (Thermo Fischer Scientific). For whole transcriptome libraries (mRNA+sRNA), cDNA libraries were prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies) including a prior step of ribodepletion using specific probes adapted to N. meningitidis (Low Input Ribo Minus Eukaryote System v2) and size fractionation of RNA prior to cDNA synthesis with RNase III was performed. Briefly, 500 ng of total RNA were ribodepleted and then fragmented. After fragmentation, mean size of fragment was around 120 nt. Coding_and_NonCoding_R1, Coding_and_NonCoding_R2, and Coding_and_NonCoding_R3 represent three technical replicates.