Single cell RNA-seq analysis for normal lymphocytes and IRF4-driven zebrafish tumors

Single cell RNA-seq analysis was carried out for 1 normal sample from a control zebrafish line (lck-mCherry) and 5 non-tumor and 14 tumor samples from the IRF4 transgenic line (lck-IRF4). Tumor cells were harvested from the animals with or without heterozygous p53 mutation at different time points of tumor development. Overall design: Human IRF4 gene was overexpressed together with mCherry under the zebrafish lck promoter. Samples were harvested from control or IRF4-transgenic zebrafish (lck-IRF4) with or without heterozygous p53 mutation. mCherry positive cells sorted by flow cytometry. Normal cells was prepared from a control zebrafish which overexpresses only mCherry. Single cell RNA-seq analysis was performed by 10X Genomics using 3' Chromium Single Cell Technique.