Datasets for benchmarking of two-pass alignment

This repository contains the datasets for benchmarking of a two-pass alignment method for long reads using the snakemake pipeline which can be found on github at https://github.com/bartongroup/two_pass_alignment_pipeline The datasets contained here are genuine nanopore DRS datasets re-basecalled from Parker et al 2020 and Workman et al. 2019. There are also simulated alignments which are each created using the error profiles of a single genuine replicate .本仓库收录了用于长读长双通路比对（two-pass alignment）方法基准测试的数据集，该基准测试所使用的Snakemake工作流可从GitHub仓库https://github.com/bartongroup/two_pass_alignment_pipeline获取。本仓库内的数据集均为源自Parker等人2020年及Workman等人2019年研究的纳米孔直接RNA测序（Nanopore Direct RNA Sequencing, DRS）真实数据集，且已完成重新碱基识别。此外本仓库还包含模拟比对数据集，所有模拟比对数据集均基于单份真实重复样本的错误特征谱生成。