Molecular phylogeny of Triatomini (Hemiptera: Reduviidae: Triatominae)

Background: The Triatomini and Rhodniini (Hemiptera: Reduviidae) tribes include the most diverse Chagas disease vectors; however, the phylogenetic relationships within the tribes remain obscure. This study provides the most comprehensive phylogeny of Triatomini reported to date. Methods: The relationships between all of the Triatomini genera and representatives of the three Rhodniini species groups were examined in a novel molecular phylogenetic analysis based on the following six molecular markers: the mitochondrial 16S; Cytochrome Oxidase I and II (COI and COII) and Cytochrome B (Cyt B); and the nuclear 18S and 28S. Results: Our results show that the Rhodnius prolixus and R. pictipes groups are more closely related to each other than to the R. pallescens group. For Triatomini, we demonstrate that the large complexes within the paraphyletic Triatoma genus are closely associated with their geographical distribution. Additionally, we observe that the divergence within the spinolai and fl...研究背景：半翅目（Hemiptera）猎蝽科（Reduviidae）下的锥蝽族（Triatomini）与鼻猎蝽族（Rhodniini）是恰加斯病（Chagas disease）多样性最高的传播媒介类群；然而，该两族内部的系统发育关系仍不明朗。本研究构建了当前已有报道中最为全面的锥蝽族系统发育树。 研究方法：本研究基于6种分子标记开展全新的分子系统发育分析，以此解析所有锥蝽族属级类群与3个鼻猎蝽族物种类群代表类群间的系统发育关系。所用分子标记包括：线粒体16S rRNA基因、细胞色素氧化酶I与II（Cytochrome Oxidase I and II，简称COI、COII）、细胞色素B（Cytochrome B，简称Cyt B），以及核基因18S与28S rRNA基因。 研究结果：结果显示，粗腿红猎蝽（Rhodnius prolixus）类群与淡足红猎蝽（R. pictipes）类群之间的亲缘关系，较二者与苍白红猎蝽（R. pallescens）类群的亲缘关系更为紧密。针对锥蝽族，本研究证实并系群锥蝽属（Triatoma）内的多个大型复合类群与其地理分布高度相关。此外，本研究还观察到spinolai类群与fl[...]的遗传分化