Transcriptome analysis of synthetic miR-155 oligo-treated mouse CD4+ T-cells

High-density gene expression profile of 20,000+ genes for synthetic miR-155 oligo-treated mouse CD4+ T-cells using RNA deep sequencing technology. Mouse CD4+ T-cells were purified with a CD4+ T-cell isolation kit (Miltenyi Biotec, Shanghai, China) and by magnetic bead separation. Cell purity was confirmed by flow cytometry. A total of 3 × 104 cells were transferred by synthetic miR-155 oligo. At 8 h post transfection, cells were collected and total RNA was isolated. Sequencing libraries were prepared with an Illumina Truseq RNA sample preparation kit according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Single-read sequencing (read length: 50 bp) was conducted on an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA).