RNA-seq comparing UVB and poly(I:C) treated keratinocytes

TLR3 is expressed in human skin and keratinocytes and given its varied role in skin inflammation, development, and regeneration, we sought to determine the cellular response in normal human keratinocytes (NHEKs) to TLR3 activation. We investigated this mechanism by treating primary human keratinocytes with both UVB, an endogenous and physiologically relevant TLR3 activator, and poly(I:C), a synthetic and selective TLR3 ligand. NHEKs were exposed to a dose of 10 mJ/cm2 UVB or to 20 ug/mL of poly(I:C) for 24 hours. Control cells received no UVB or poly(I:C) treatment. Cells were harvested 72 hours after initial plating. Three replicates/group were collected for a total of 12 samples.