This fileset contains single-molecule FRET datasets used as demonstrative dataset for the FRETBursts software (https://github.com/tritemio/FRETBursts). These datasets come from smFRET experiments on a
Energy transfer proceeds in orthogonal dyads in contrast to Förster’s theory and cannot be prohibited even by rigid interconnecting cage compounds such as cubane or diamantane.
Donor- and acceptor-labelled GpA TM (each 0.25 µM) domains were separately solubilized in 20 µM APol at a final polymer/peptide ratio of 40∶1. (A) After incubation at 37°C, donor and acceptor were mix
This Excel spreadsheet, includes the results of calculation of overlap integral and R0 values for all possible combinations of TA and NTD labeled strands (using the data from steady-state fluorescence
