Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging

Description This dataset contains spatial transcriptomics from CosMx platform, for the paper entitled "Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging." In brief, this paper presents a system that enables in vivo heterochronic myeloid replacement and identified a key signaling pathway involved in aging microglia in the brain. The compressed files contain the raw seurat object output from AtoMx Bioinformatics Platform, provided by Nanostring. Additional raw imaging data or additional metadata for CosMx are available upon request. Data Manifest Filename Contained Objects Treatment Description Associated Figures CosMx Panel Run1_Young_Old_June_2024.tar.gz seuratObject_Young_June_2024.RDS Young HSC into Young animal, replicate #1 Fig. 2; Fig. S4 Mouse 1k Universal Run1_Young_Old_June_2024.tar.gz seuratObject_Old_June_2024.RDS Young HSC into Old animal, replicate #1 Fig. 2; Fig. S4 Mouse 1k Universal Run2_Young_Old_June_2024.tar.gz seuratObject_Young_June_2024.RDS Young HSC into Young animal, replicate #2 Fig. 2; Fig. S4 Mouse 1k Universal Run2_Young_Old_June_2024.tar.gz seuratObject_Old_June_2024.RDS Young HSC into Old animal, replicate #2 Fig. 2; Fig. S4 Mouse 1k Universal Run3_IFN_Young_Old_Mar_2024.tar.gz seuratObject_young_12_IFN_Panel.RDS Young HSC into Young animal, replicate #1 Fig. 3; Fig. S7 Mouse 1k Universal with IFN/Aging gene panel Run3_IFN_Young_Old_Mar_2024.tar.gz seuratObject_old_3_IFN_Panel.RDS Young HSC into Old animal, replicate #1 Fig. 3; Fig. S7 Mouse 1k Universal with IFN/Aging gene panel Run4_IFN_Young_Old_Mar_2024.tar.gz seuratObject_young_14_IFN_Panel.RDS Young HSC into Young animal, replicate #2 Fig. 3; Fig. S7 Mouse 1k Universal with IFN/Aging gene panel Run4_IFN_Young_Old_Mar_2024.tar.gz seuratObject_old_5_IFN_Panel.RDS Young HSC into Old animal, replicate #2 Fig. 3; Fig. S7 Mouse 1k Universal with IFN/Aging gene panel Run5_Stat1_study_2025.tar.gz seuratObject_7oc_brain.RDS Control Young HSC into Old animal, replicate #1 Fig.4; Fig. S11 Mouse 1k Universal with IFN/Aging gene panel Run5_Stat1_study_2025.tar.gz seuratObject_4oko_brain.RDS Stat1 -/- Young HSC into Old animal, replicate #1 Fig.4; Fig. S11 Mouse 1k Universal with IFN/Aging gene panel Run5_Stat1_study_2025.tar.gz seuratObject_8oko_brain.RDS Stat1 -/- Young HSC into Old animal, replicate #2 Fig.4; Fig. S11 Mouse 1k Universal with IFN/Aging gene panel数据集说明 本数据集包含来自CosMx平台（CosMx platform）的空间转录组数据，配套发表的论文标题为《异时性髓系细胞置换揭示局部脑环境是小胶质细胞衰老的关键驱动因素》。简言之，本研究搭建了一套可实现体内异时性髓系置换的实验系统，并鉴定出大脑中参与小胶质细胞衰老的关键信号通路。压缩包内包含由Nanostring公司提供的AtoMx生物信息学平台（AtoMx Bioinformatics Platform）输出的原始Seurat对象（Seurat object）文件。如需获取CosMx的额外原始成像数据或补充元数据，可提交申请。 数据清单 | 文件名 | 包含对象 | 处理方案说明 | 关联图表 | CosMx检测面板 | | ---- | ---- | ---- | ---- | ---- | | Run1_Young_Old_June_2024.tar.gz | seuratObject_Young_June_2024.RDS | 年轻造血干细胞（Hematopoietic Stem Cell, HSC）移植至年轻动物，重复实验1 | 图2；图S4 | Mouse 1k Universal | | Run1_Young_Old_June_2024.tar.gz | seuratObject_Old_June_2024.RDS | 年轻造血干细胞（HSC）移植至老年动物，重复实验1 | 图2；图S4 | Mouse 1k Universal | | Run2_Young_Old_June_2024.tar.gz | seuratObject_Young_June_2024.RDS | 年轻造血干细胞（HSC）移植至年轻动物，重复实验2 | 图2；图S4 | Mouse 1k Universal | | Run2_Young_Old_June_2024.tar.gz | seuratObject_Old_June_2024.RDS | 年轻造血干细胞（HSC）移植至老年动物，重复实验2 | 图2；图S4 | Mouse 1k Universal | | Run3_IFN_Young_Old_Mar_2024.tar.gz | seuratObject_young_12_IFN_Panel.RDS | 年轻造血干细胞（HSC）移植至年轻动物，重复实验1 | 图3；图S7 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run3_IFN_Young_Old_Mar_2024.tar.gz | seuratObject_old_3_IFN_Panel.RDS | 年轻造血干细胞（HSC）移植至老年动物，重复实验1 | 图3；图S7 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run4_IFN_Young_Old_Mar_2024.tar.gz | seuratObject_young_14_IFN_Panel.RDS | 年轻造血干细胞（HSC）移植至年轻动物，重复实验2 | 图3；图S7 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run4_IFN_Young_Old_Mar_2024.tar.gz | seuratObject_old_5_IFN_Panel.RDS | 年轻造血干细胞（HSC）移植至老年动物，重复实验2 | 图3；图S7 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run5_Stat1_study_2025.tar.gz | seuratObject_7oc_brain.RDS | 对照：年轻造血干细胞（HSC）移植至老年动物，重复实验1 | 图4；图S11 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run5_Stat1_study_2025.tar.gz | seuratObject_4oko_brain.RDS | Stat1基因敲除（Stat1 -/-）年轻造血干细胞（HSC）移植至老年动物，重复实验1 | 图4；图S11 | 搭载干扰素/衰老基因面板的Mouse 1k Universal | | Run5_Stat1_study_2025.tar.gz | seuratObject_8oko_brain.RDS | Stat1基因敲除（Stat1 -/-）年轻造血干细胞（HSC）移植至老年动物，重复实验2 | 图4；图S11 | 搭载干扰素/衰老基因面板的Mouse 1k Universal |