Non-drive allele frequency dynamics in Ag(QFS)1 gene drive caged population experiments pre-seeded with R1 or R3 resistant alleles in Anopheles gambiae

This dataset contains paired-read Illumina pooled amplicon sequencing data tracking non-drive allele frequencies over discrete generations in Anopheles gambiae cage population experiments with the Ag(QFS)1 gene drive, pre-seeded with known resistance alleles at the doublesex gene drive target site (an R1 G→T SNP or R3 C→T SNP). Ag(QFS)1 heterozygous males were released at 40% (R1 cages) and 25% (R3 cages) frequency in duplicate cages pre-seeded with 15% R1 (G→T SNP) and 25% R3 (C→T SNP) resistant alleles respectively. File naming denotes whether the sample originated from an R1 or R3 pre-seeded cage trial, the generation sampled (G0, G1, G4 or G8), the cage sampled (A, B, C or D) and the population batch sampled (1 or 2). Cage A: population pre-seeded with R3 (replicate 1) Cage B: population pre-seeded with R3 (replicate 2) Cage C: population pre-seeded with R1 (replicate 1) Cage D: population pre-seeded with R1 (replicate 2)本数据集包含双端测序读段（paired-read）Illumina混合扩增子测序数据，用于追踪携带Ag(QFS)1基因驱动（gene drive）的冈比亚按蚊（Anopheles gambiae）笼养种群实验中，离散世代下的非驱动等位基因频率；该种群预先在双性基因（doublesex）基因驱动靶位点处引入了已知抗性等位基因，包括R1型G→T单核苷酸多态性（Single Nucleotide Polymorphism, SNP）或R3型C→T单核苷酸多态性（SNP）。 将Ag(QFS)1杂合雄性蚊虫分别以40%（R1组笼）与25%（R3组笼）的释放频率，接种至预先分别携带15% R1（G→T SNP）与25% R3（C→T SNP）抗性等位基因的重复笼养种群中。 文件名编码规则可指示样本来源的核心信息，具体涵盖：预先接种R1或R3的笼养试验组别、采样世代（G0、G1、G4或G8）、采样笼盒（A、B、C或D）以及采样种群批次（1或2）。 各笼盒对应种群信息如下： 笼盒A：预先接种R3抗性等位基因的种群（生物学重复1） 笼盒B：预先接种R3抗性等位基因的种群（生物学重复2） 笼盒C：预先接种R1抗性等位基因的种群（生物学重复1） 笼盒D：预先接种R1抗性等位基因的种群（生物学重复2）