Prediction of broadly neutralizing antibodies [10X]

We generated the humoral immune repertoire of circulating memory B cells from healthy and HIV infected immune donors. These repertoires were used to predict specific HIV antibodies directed against gp120/gp41. Overall design: Peripheral blood mononuclear cells (PBMC) were isolated from one healthy donor (D1) , 1 HIV infected donor (D2) and 2 pools of HIV infected donor (G3 and G4). Single-cell BCR repertoire sequencing of CD19+ cells, for each donor/pool, was performed using Single Cell V(D)J Reagent Kit v2 from 10X Genomics.