Raw dataset of seminal proteomic from obesity and diabetes rats

Study question: The obesity and diabetes induced in a male rodent can change proteome of spermatozoa?

What data shows: These data show the sperm cells profile proteomic of 30 male Wistar rats, separated into 3 groups, control (n = 10), obese (n = 10) and diabetics (n = 09). Rats were fed with commercial food provided controllably to control group and diabetic group or hypercaloric diet added 5% sucrose in ad libitum water to obese group, for 38 weeks. The diabetic group was induced with 35 mg / kg of streptozotocin, 53 days before euthanasia, respecting the spermatogenesis cycle. After euthanasia, sperm were collected and were separated of the seminal plasma by centrifugation and prepared individually for proteomic analysis by ESI Q-Tof mass spectrometer.

How the data can be interpreted: There are three folders in the dataset, separated by control, diabetic and obese groups, with all quantified sperm cell proteins, per animal, properly identified.

研究问题：雄性啮齿动物罹患肥胖与糖尿病是否会改变其精子的蛋白质组？

数据集内容：本数据集包含30只雄性Wistar大鼠的精子蛋白质组学图谱，实验动物被分为3组：对照组（n=10）、肥胖组（n=10）与糖尿病组（n=9）。实验周期共计38周：对照组与糖尿病组大鼠饲喂定量商业饲料，肥胖组大鼠则饲喂高热量饲料，并在饮水中添加5%蔗糖以供自由采食。糖尿病组于安乐死53天前，以35mg/kg剂量的链脲佐菌素（streptozotocin）诱导糖尿病，造模时序契合精子发生周期。安乐死后，研究人员收集精子样本，通过离心分离精浆，随后将每份样本单独制备，采用电喷雾电离-四极杆飞行时间（ESI Q-Tof）质谱仪开展蛋白质组学分析。

数据集解读说明：本数据集包含三个文件夹，分别对应对照组、糖尿病组与肥胖组，内含每只实验动物的全部已定量且经准确鉴定的精子细胞蛋白质数据。