Barcoding populations of Pseudomonas fluorescens SBW25

In recent years evolutionary biologists have developed increasing interest in the use of barcoding strategies to study eco-evolutionary dynamics of lineages within evolving populations and communities. Although barcoded populations can deliver unprecedented insight into evolutionary change, barcoding microbes presents specific technical challenges. Here, strategies are described for barcoding populations of the model bacterium Pseudomonas fluorescens SBW25, including the design and cloning of barcoded regions, preparation of libraries for amplicon sequencing, and quantification of resulting barcoded lineages. In so doing, we hope to aid the design and implementation of barcoding methodologies in a broad range of model and non-model organisms. In here we deposit, the raw and processed data we have used during our study.近年来，进化生物学家对利用条形码（barcoding）策略探究进化种群与群落内谱系的生态进化动力学的兴趣日益浓厚。尽管经条形码标记的种群可为进化变化研究提供前所未有的洞见，但对微生物开展条形码标记仍存在特定的技术挑战。本研究详述了模式细菌荧光假单胞菌（Pseudomonas fluorescens）SBW25的种群条形码标记方案，涵盖条形码标记区域的设计与克隆、扩增子测序文库的制备，以及所得标记谱系的定量分析。借此，我们期望可为诸多模式与非模式生物的条形码标记方法的设计与实施提供助力。本文同时上传了本研究中使用的原始数据与经处理的数据。