Code for JupyterNotebook for scRNA-seq data analysis_Oviduct Pgr cKO and P4-treated WT mice

Codes for single-cell RNA-sequencing analysis of data from mouse oviducts. WT and cKO data were collected from Pgrf/f (WT) or Wnt7aCre/+;Pgrf/f (cKO) at 0.5 days post coitus (dpc) another dataset was collected from C57BL6/J adult mice that were ovariectomized and treated with progesterone (P4) at 1 mg/mouse for 2 or 24 hours compared to vehicle control. The oviducts were dissected into infundibulum+ampulla (InfAmp or IA) and isthmus+UTJ (IsthUTJ or IU) regions.

本代码用于小鼠输卵管数据集的单细胞RNA测序（single-cell RNA-sequencing）分析。野生型（WT）与条件性敲除（cKO）数据集采集自交配后0.5天（days post coitus, dpc）的Pgrf/f（WT）或Wnt7aCre/+;Pgrf/f（cKO）小鼠；另一数据集采集自成年C57BL6/J小鼠，该类小鼠先接受去卵巢手术，随后以每只1mg的剂量注射孕酮（progesterone, P4）处理2小时或24小时，并以溶剂对照组（vehicle control）作为对照。实验中将输卵管解剖为漏斗部+壶腹部（InfAmp或IA）以及峡部+子宫输卵管连接部（IsthUTJ或IU）两个区域。