Transcriptional profiling of Hepa1-6-bearing mouse model

Hepa1-6-bearing C57BL/6 mouse model was established to evaluate the therapeutic efficacy of Ganoderma lucidum extract (GLE) in HCC. The GLE treated and untreated mice were used for transcriptioanl profiles detection, and 126 differentially expressed lncRNAs and 558 DE mRNAs were identified, respectively. The bioinformatics analysis shows that the GLE could suppress the growth and proliferation of HCC by PI3K/Akt/mTOR and MAPK signaling pathway, and also could induce apoptosis of tumor cell by mitochondrial and death receptor pathway. 6 samples, including 3 GLE treated Hepa1-6-bearing C57 BL/6 mice and 3 GLE untreated Hepa1-6-bearing C57 BL/6 mice were performed to analyze