16S analysis of the gut microbiota from high-fat fed mice treated with Prevotella copri. Prevotella copri

Mice (24 male WT C57BL/6J, Taconic, 10 weeks of age) were housed in cages of four and kept at 22°C in a specific pathogen free facility under a 12 hour light-dark cycle, fed ad libitum and had free access to water. After 2 weeks of acclimatization on a standard chow diet (Altromin 1310, Altromin, Germany), mice were transferred to a soy protein-based 60% high-fat diet low in BCAAs (12492D, Ssniff, Germany) in order to prime for later development of glucose intolerance and insulin resistance and sham gavaged (1% glycerol in PBS) twice a week. After three weeks on high-fat diet, mice were divided into two equal groups of 12 mice each stratified by magnetic resonance (MR)-based fat mass and weight. For the following three weeks, mice were gavaged twice weekly with 100 µl of either P. copri (5 × 108 CFU/mouse, 1% glycerol in PBS) or sham while remaining on the high-fat diet.