Data for: Age and an obesogenic diet affect mouse behaviour in a sex-dependent manner

Obesity is rising globally and is associated with neurodevelopmental and psychiatric disorders among children, adolescents, and young adults. Whether obesity is the cause or the consequence of these disorders remains unclear. To examine the behavioural effects of obesity systematically, locomotion, anxiety, and social behaviour were assessed in male and female C57Bl/6J mice using the open field, elevated plus maze and social preference task. First, the effects of age and sex were examined in control mice, before investigating post-weaning consumption of a high fat, high sugar diet commonly consumed in human populations with high rates of obesity. In the open field and elevated plus maze, locomotor activity and anxiety-related behaviours reduced with aging in both sexes, but with different sex-specific profiles. The high fat, high sugar diet reduced food and calorie intake and increased body mass and fat deposition in both sexes. In the open field, both male and female mice on the obesog..., Behavioural data from the open field, elevated plus maze and social preference tasks was collected using a ceiling mounted webcam over the testing apparatus followed by manual experimenter counting, blind to cohort where possible.
Food, calorie and protein intake for a cage of mice was determined by measuring food weekly over a 24 hour period and expressed as intake per day per gram of the total weight of mice in each cage.
Body weight of individual mice was monitored weekly from weaning to the end of the experimental period. 
A subset of the females and males in the two diet groups were used to quantify total body fat and lean mass by Dual Energy X-ray Absorptiometry (DEXA) scanning (Lunar PIXImus densitometer; GE Healthcare) and/or to measure the weights of the individual fat deposits and body organs.
All data sets were added to an Excel spreadsheet and statistical comparisons between groups were made using GraphPad Prism (version 9).,

全球范围内肥胖患病率持续上升，且与儿童、青少年及青年群体的神经发育障碍和精神疾病密切相关。目前尚不明确肥胖是此类疾病的诱因还是结果。为系统探究肥胖对行为学的影响，本研究采用旷场实验(open field)、高架十字迷宫(elevated plus maze)及社交偏好任务(social preference task)，对雌雄C57Bl/6J小鼠的运动活性、焦虑相关行为及社交行为进行评估。首先，本研究先对对照组小鼠的年龄与性别效应进行检测，随后探究断奶后喂食高脂高糖饲料（该类饲料在肥胖患病率较高的人群中较为常见）对小鼠的影响。在旷场实验与高架十字迷宫中，雌雄小鼠的运动活性及焦虑相关行为均随年龄增长而下降，但二者的性别特异性变化模式存在差异。高脂高糖饲料可降低雌雄小鼠的食物与热量摄入，并增加其体质量与脂肪沉积量。在旷场实验中，喂食致肥性[原文表述截断]饲料的雌雄小鼠……。旷场实验、高架十字迷宫及社交偏好任务的行为学数据，通过安装于测试装置上方的壁挂式网络摄像头进行采集，随后由实验人员进行人工计数，尽可能对实验分组设盲以避免偏倚。
每笼小鼠的食物、热量及蛋白质摄入量，通过每周连续24小时测量食物消耗量进行测定，并以每笼小鼠总重量对应的每日摄入量进行表达。
从断奶至实验结束，每周监测每只小鼠的体质量。
本研究选取两个饲料组中的部分雌雄小鼠，通过双能X射线吸收法(Dual Energy X-ray Absorptiometry, DEXA)扫描（使用GE Healthcare的Lunar PIXImus骨密度仪）对其全身脂肪与瘦体重进行定量分析，同时或单独测量各脂肪沉积部位及身体器官的重量。
所有数据集均录入Excel电子表格，组间统计学比较采用GraphPad Prism（第9版）完成。