Additional file 1 – Enzyme activity assay excel template

p { margin-bottom: 0.25cm; direction: ltr; line-height: 115%; text-align: left; }p.western { font-size: 11pt; }p.cjk { font-size: 11pt; }a:link { color: rgb(0, 0, 255); } The file contains a blank layout sheet for test sample and control sample distribution on a 96-well plate. Additional sheets contain master mix volume calculations for activity assays for Aldolase, Glucose-6-phosphate Dehydrogenase, Hexokinase, Phosphofructokinase, Phosphoglucose isomerase, and Phosphoglucosemutase. Adjustment of the desired number of control and test samples (grey fields) results in recalculation of required volumes. All sheets are printable on A4 paper and can be filled out digitally or manually.