Loss of Musashi-1 inhibits breast cancer metastasis via regulation of Timp3/Mmp9

To investigate the molecular mechanism of Msi1 in breast cancer metastasis, we performed transcriptome profiling at 16 weeks of primary tumor samples between ctrl and Msi1 cko mice, We performed a pathway enrichment analysis of the transcriptome data, the enriched KEGG pathways are mostly related to cellular adhesion, such as Focal adhesion, cell adhesion molecular (CAM), ECM-receptor interaction and regulation of actin cytoskeleton, which are closely related to the formation of invadopodia. Overall design: mammary tumor mRNA profiles of 16 weeks of ctrl and Msi1 cko mice were generated by deep sequencing, using Illumina NovaSeq 6000 platform.